PUM2 Antibody

SKU: orb19016

Description

Goat polyclonal antibody to PUM2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:4000, WB: 0.3-0.5 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 110kDa band observed in Human Epstein-Barr virus immortilised lymphoblastoid lysates (calculated MW of 114kDa according to NP_056132.1). Recommended concentration: 1-3 μg/ml. NB: Some minor bands are resistant to blocking by the immunizing peptide.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PUM2
• Protein Sequence: DQNRDLKQGDDDDSK
• Molecular Weight: 114
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PUM2 antibody, anti PUMH2 antibody, anti PUML2 antibody, anti KIAA0235 antibody, anti pumilio homolog 2 (Drosophila) antibody, anti pumilio (Drosphila) homolog 2 antibody, anti FLJ36528 antibody, anti MGC138251 antibody, anti MGC138253 antibody, anti OTTHUMP00000200539 antibody, anti pumilio homolog 2 antibody

Western blot analysis of Human Epstein-Barr virus immortilised lymphoblastoid lysate using PUM2 antibody

Western blot analysis of HEK293 lysate using PUM2 antibody

Immunohistochemical staining of Human Testis using PUM2 antibody

Quick Database Links

Gene Symbol

PUM2

NCBI Gene ID

23369