PU.1 Antibody / SPI1

• Catalog Number: orb534180
• Category: Antibodies
• Description: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity). [UniProt]
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 16-170 from the human protein was used as the immunogen for the PU.1 antibody.
• UniProt ID: P17947
• Tested applications: IF, IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Application notes: The stated application concentrations are suggested starting points. Titration of the PU.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Antibody Type: Primary Antibody
• Clone Number: TFPU1-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot testing of human THP-1 cell lysate with PU.1 antibody (clone TFPU1-1). Predicted molecular weight ~31 kDa but routinely observed at ~40 kDa.

IHC staining of FFPE human lymph node with PU.1 antibody (clone TFPU1-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC staining of FFPE human Hodgkin's lymphoma with PU.1 antibody (clone TFPU1-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human Ramos cells with PU.1 antibody (green, clone TFPU1-1) and Phalloidin (red).