PU.1 Antibody / SPI1

• Catalog Number: orb534180
• Category: Antibodies
• Description: Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity). [UniProt]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: TFPU1-1
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 16-170 from the human protein was used as the immunogen for the PU.1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This PU.1 antibody is available for research use only.
• UniProt ID: P17947
• Storage: Store the PU.1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The stated application concentrations are suggested starting points. Titration of the PU.1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Expiration Date: 12 months from date of receipt.