PU.1 Antibody / SPI1

• Catalog Number: orb1151709
• Category: Antibodies
• Description: Mouse monoclonal antibody to PU.1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 16-170 was used as the immunogen for the PU.1 antibody.
• UniProt ID: P17947
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the PU.1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PU1/2118
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This PU.1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human colon carcinoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Hodgkins Lymphoma tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human Lymph Node tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human spleen tissue with PU.1 antibody (clone PU1/2118). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free PU.1 antibody (clone PU1/2118) as confirmation of integrity and purity.