PTPN11 Antibody

SKU: orb1274520

Description

PTPN11 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, KO/KD Validated, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-460 of human PTPN11 (NP_002825.3).
• Target: PTPN11
• Molecular Weight: Observed: 75kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SHP2 Antibody: CFC, NS1, SHP2, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP3, SHPTP2, Tyrosine-protein phosphatase non-receptor type 11, Protein-tyrosine phosphatase 1D

Western blot analysis of extracts of various cell lines, using PTPN11 antibody (orb1274520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunofluorescence analysis of C6 cells using PTPN11 antibody (orb1274520) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts from normal (control) and PTPN11 knockout (KO) 293T cells, using PTPN11 antibody (orb1274520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Quick Database Links

Gene Symbol

PTPN11

UniProt

Q06124