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Catalog Number | orb18653 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PTPN11 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, WB |
Reactivity | Bovine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10ug/ml |
Conjugation | Unconjugated |
MW | 68.0; 68.4 |
Target | SHP2 / PTPN11 |
Entrez | 5781 |
Protein Sequence | YENVGLMQQQKSFR |
RRID | AB_10750729 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PTPN11 antibody, anti SHP2 antibody, anti pro Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in Human Skeletal Muscle lysates and in lysates of cell line HEK293 (calculated MW of 68.0kDa according to NP_002825.3). In transfected HEK293 transiently expressing PTPN11 bands of approx. 70kDa and 49kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended for use at 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human muscle lysate using PTPN11 antibody
Immunohistochemical staining of Human Cerebellum using PTPN11 antibody
Immunohistochemical staining of Human Kidney using PTPN11 antibody
Flow cytometric analysis of paraformaldehyde fixed A431 cells using PTPN11 antibody.
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using PTPN11 antibody.