SHP2/PTPN11 Antibody

SKU: orb18653

Description

Goat polyclonal antibody to PTPN11

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in Human Skeletal Muscle lysates and in lysates of cell line HEK293 (calculated MW of 68.0kDa according to NP_002825.3). In transfected HEK293 transiently expressing PTPN11 bands of approx. 70kDa and 49kDa are observed. These bands are not observed in the non-transfected HEK293. Recommended for use at 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SHP2 / PTPN11
• Molecular Weight: 68.0; 68.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti PTPN11 antibody, anti SHP2 antibody, anti protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1) antibody, anti CFC antibody, anti NS1 antibody, anti BPTP3 antibody, anti PTP2C antibody, anti SHP-2 antibody, anti PTP-1D antibody, anti SH-PTP2 antibody, anti SH-PTP3 antibody, anti Noonan syndrome 1 antibody, anti protein-tyrosine phosphatase 2C antibody, anti protein tyrosine phosphatase, non-receptor type 11 antibody, anti MGC14433 antibody, anti protein tyrosine phosphatase-2 antibody

Western blot analysis of human muscle lysate using PTPN11 antibody

Immunohistochemical staining of Human Cerebellum using PTPN11 antibody

Immunohistochemical staining of Human Kidney using PTPN11 antibody

Flow cytometric analysis of paraformaldehyde fixed A431 cells using PTPN11 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using PTPN11 antibody.

Quick Database Links

Gene Symbol

SHP2 / PTPN11

NCBI Gene ID

5781