PTCH Antibody

• Catalog Number: orb1247056
• Category: Antibodies
• Description: PTCH Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Predicted Reactivity: Bovine, Canine, Porcine
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: C-HPESRHHPPSNPRQQ
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PTCH
• UniProt ID: Q13635
• NCBI: NP_001077075.1, NP_000255.2, NP_001077072.1, NP_001077071.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PTCH, patched homolog (Drosophila), RP11-435O5.3,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 150kDa band observed in Human Brain lysates (calculated MW of 153.9kDa according to NP_001077071.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence :Expression of the protein seen in the Golgi/membrane of NIH3T3 cells. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247056 (1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247056 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing Golgi/membrane staining.