PTCH1 (aa266-280) Antibody

SKU: orb107648

Description

Goat polyclonal antibody to PTCH1

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  WB: Approx 140kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159kDa according to NP_032983.1). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 8µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PTCH1 (aa266-280)
• Protein Sequence: EELKKINYQVDSWEE
• Molecular Weight: 161; 161; 154; 144
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PTCH1 antibody, anti patched 1 antibody, anti BCNS antibody, anti FLJ26746 antibody, anti FLJ42602 antibody, anti HPE7 antibody, anti NBCCS antibody, anti PTC antibody, anti PTC1 antibody, anti PTCH11 antibody, anti OTTHUMP00000021709 antibody, anti OTTHUMP00000021710 antibody, anti PTCH protein +12b antibody, anti PTCH protein +4 antibody, anti PTCH protein-10 antibody, anti patched homolog 1 antibody, anti protein patched homolog 1 antibody

Western blot analysis of Mouse fetal Kidney lysate (35ug protein in RIPA buffer) using PTCH1 antibody

Immunofluorescence analysis of HeLa cells using PTCH1 antibody

Quick Database Links

Gene Symbol

PTCH1 (aa266-280)

NCBI Gene ID

5727