KLK3 Antibody

SKU: orb1252646

Description

KLK3 Antibody

Research Area

Cancer Biology, Cell Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Application Notes:
  ELISA: For coating use Ab at 1-5 µg/mL; order Ab without BSAFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLIHC (FFPE): 0.5-1.0 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Variations in protocols, secondaries and substrates may require the PSA antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone No.: KLK3/801
• Immunogen: Full length human recombinant protein was used as the immunogen for this PSA antibody.
• Target: KLK3
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Prostate-specific antigen, PSA, Gamma-seminoprotein, Seminin, Kallikrein-3, P-30 antigen, Semenogelase, KLK3, APS

PSA antibody IHC testing of formalin-paraffin human prostate carcinoma.

SDS-PAGE Analysis of Purified, BSA-Free PSA Antibody (KLK3/801). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

Gene Symbol

KLK3

UniProt

P07288