Prostate Specific Antigen Mouse Monoclonal Antibody

• Catalog Number: orb1152046
• Category: Antibodies
• Description: Prostate Specific Antigen Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4C4
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Prostate Specific Antigen (PSA) Antigen, Native
• Antibody Type: Primary Antibody
• Dilution range: WB=1:500-2000, IHC-P=1:400-800, IHC-F=1:400-800, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: KLK3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4).
• Alternative names: antigen, prostate-specific; APS; gamma seminoprote
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Measured by its binding ability in a indirect ELISA. Immobilized Human PSA (Native) at 2 µg/mL (100 µl/well) can bind Anti-Human PSA monoclonal antibody, the minimum detection concentration is 0.1 ng/ml.

Paraformaldehyde-fixed, paraffin embedded (Human Prostate Tumor), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Prostate Specific Antigen) Monoclonal Antibody, Unconjugated (orb1152046) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Prostate Specific Antigen) Monoclonal Antibody, Unconjugated (orb1152046) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human Prostate Specific Antigen protein, Primary: Anti-Prostate Specific Antigen (orb1152046) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 23 kDa, Observed band size: 35 kDa.

Sample: Lane 1: Prostate Specific Antigen (PSA) Protein at 500 ng, Primary: Mouse Anti-PSA Monoclonal Antibody at 1/1000 dilution (Cat. orb1152046), Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 34kD, Observed band size: 32kD.