PRPF19 Antibody

• Catalog Number: orb1260836
• Category: Antibodies
• Description: PRPF19 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRPF19 (NP_055317.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 55kDa
• Target: PRPF19
• UniProt ID: Q9UMS4
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: PRPF19, NMP200, PRP19, PSO4, UBOX4, hPSO4, SNEV
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using PRPF19 antibody (orb1260836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat liver using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug PRPF19 antibody (orb1260836). Western blot was performed from the immunoprecipitate using PRPF19 antibody (orb1260836) at a dilution of 1:1000.