PRPF19 Antibody

SKU: orb1260836

Description

PRPF19 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRPF19 (NP_055317.1).
• Target: PRPF19
• Molecular Weight: Observed: 55kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

PRPF19, NMP200, PRP19, PSO4, UBOX4, hPSO4, SNEV

Western blot analysis of extracts of various cell lines, using PRPF19 antibody (orb1260836) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat liver using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using PRPF19 antibody (orb1260836) at dilution of 1:100 (40x lens).

Immunoprecipitation analysis of 200 ug extracts of Jurkat cells, using 3 ug PRPF19 antibody (orb1260836). Western blot was performed from the immunoprecipitate using PRPF19 antibody (orb1260836) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

PRPF19

UniProt

Q9UMS4