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proteinase 3 / myeloblastin Antibody

Catalog Number: orb1248285

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1248285
CategoryAntibodies
Descriptionproteinase 3 / myeloblastin Antibody
Targetproteinase 3 / myeloblastin
ClonalityPolyclonal
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceLiquid
Concentration500 ug/mL
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ImmunogenThe immunogen for this antibody is: C-HNVRTQEPTQQ
UniProt IDP24158
MWApprox 27kDa
Tested applicationsELISA, WB
Application notesPeptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 28kDa band observed in Human Spleen lysates (calculated MW of 27.8kDa according to NP_002768.3). Recommended concentration:0.3-1ug/ml. Primary incubation was 1 hour
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesPRTN3, proteinase 3, ACPA, AGP7, C-ANCA, CANCA, MB
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NoteFor research use only
NCBINP_002768.3
proteinase 3 / myeloblastin Antibody

orb1248285 (0.3 ug/ml) staining of Human Spleen lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

proteinase 3 / myeloblastin Antibody

orb1248285 (5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.