Goat anti-proteinase 3 / myeloblastin (aa88-98) Antibody

• Catalog Number: orb167717
• Category: Antibodies
• Description: Goat polyclonal antibody to PRTN3.
• Target: proteinase 3 / myeloblastin (aa88-98)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HNVRTQEPTQQ
• MW: 27,8
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 28kDa band observed in Human Spleen lysates (calculated MW of 27.8kDa according to NP_002768.3). Recommended concentration:0.3-1µg/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti PRTN3 antibody, anti proteinase 3 antibody, a
• Note: For research use only
• Entrez: 5657

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Primary incubation 1 hour at room temperature. Images A and B: Human Bone Marrow and Spleen lysate at primary Ab concentration 1 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).