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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 77.3 KDa. Observed: 100-120 KDa, reducing conditions
Mouse
Ser22-Arg702
C-6His
10 μg, 50 μg, 500 μg, 1 mgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 16.7 KDa. Observed: 20-25 KDa, reducing conditions
Human
Gln22-Pro152
C-Avi-6His
20 μg, 100 μgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 18.1 KDa. Observed: 30-35 KDa, reducing conditions
Human
Gln22-His163
C-Avi-6His
100 μg, 20 μgBiotin
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 16.4 KDa. Observed: 30-40 KDa, reducing conditions
Human
Gln19-Pro139
C-Avi-6His
20 μg, 100 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 52 KDa. Observed: 60-80 KDa, reducing conditions
Rhesus macaque
Leu23-Asp257
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 60.7 KDa. Observed: 80-90 KDa, reducing conditions
Human
Gly32-Val351
C-mFc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 16.1 KDa. Observed: 20-27 KDa, reducing conditions
Human
Tyr29-Gln159(R127A, R130A)
C-10His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 16.1 KDa. Observed: 20-30 KDa, reducing conditions
Rhesus macaque
Ala30-Asp161
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 64.2 KDa. Observed: 80-100 KDa, reducing conditions
Human
Met17-Ala363
C-mFc
50 μg, 500 μg, 1 mg, 10 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 35.3 KDa. Observed: 40-50 KDa, reducing conditions
Mouse
Gly31-Ile349
C-6His
10 μg, 50 μg, 500 μg, 1 mg
