Biotinylated Human CD47

• Catalog Number: orb2993805
• Category: Proteins
• Description: Biotinylated Recombinant Human Leukocyte Surface Antigen CD47 is produced by our Mammalian expression system and the target gene encoding Gln19-Pro139 is expressed with a Avi, 6His tag at the C-terminus.
• Conjugation: Biotin
• Tag: C-Avi-6His
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 10mMTris-Citrate, 150mM NaCl, pH 8.0.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• UniProt ID: Q08722
• MW: Predicted: 16.4 KDa. Observed: 30-40 KDa, reducing conditions
• Expression System: Human Cells
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Biological Origin: Human
• Biological Activity: BLI: Loaded Anti-Human CD47 mAb-Fc(Cat#orb2993150) on Protein A Biosensor, can bind Human CD47-Avi-6His(Cat#orb2993805) with an affinity constant of 0.20 nM as determined in BLI assay.
• Expression Region: Gln19-Pro139
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Leukocyte Surface Antigen CD47; Antigenic Surface
• Note: For research use only
• Expiration Date: 6 months from date of receipt.