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Catalog Number | orb629957 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to PKC iota |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, IP, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | protein kinase C, iota |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:50-1:500; IF: 1:50-1:500 |
Form/Appearance | liquid |
Purity | ≥95% as determined by SDS-PAGE |
Conjugation | Unconjugated |
MW | 68 kDa |
Target | PRKCI |
UniProt ID | P41743 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | aPKC lambda/iota, DXS1179E, PKC iota, PKCI, PRKC l Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
HeLa cells were subjected to SDS PAGE followed by western blot with orb629957 (PRKCI antibody) at dilution of 1:500.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using orb629957 (PKC iota antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).
IP Result of anti-PKC iota (IP: orb629957, 3 ug; Detection: orb629957 1:500) with HeLa cells lysate 2500 ug.
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using orb629957 (PRKCI Antibody) at dilution of 1:200.
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rabbit, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rabbit, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |