PKC iota/PRKCI Rabbit Polyclonal Antibody

SKU: orb234367

Description

PKC iota/PRKCI Rabbit Polyclonal Antibody

Research Area

Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human PKC iota recombinant protein (Position: D21-Q214). Human PKC iota shares 96% and 97% amino acid (aa) sequence identity with mouse and rat PKC iota, respectively.
• Target: Protein kinase C iota type
• Molecular Weight: 68 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein kinase C iota type; 2.7.11.13; Atypical protein kinase C-lambda/iota; PRKC-lambda/iota; aPKC-lambda/iota; nPKC-iota; PRKCI; DXS1179E

Anti-PKC iota Picoband antibody, Western blotting. All lanes: Anti PKC iota at 0.5 ug/ml. Lane 1: SHG Whole Cell Lysate at 40 ug. Lane 2: A549 Whole Cell Lysate at 40 ug. Lane 3: U87 Whole Cell Lysate at 40 ug. Lane 4: 293T Whole Cell Lysate at 40 ug. Lane 5: HELA Whole Cell Lysate at 40 ug. Lane 6: JURKAT Whole Cell Lysate at 40 ug. Predicted bind size: 68 KD. Observed bind size: 68 KD.

Anti-PKC iota Picoband antibody, IHC(P): Human Lung Cancer Tissue.

Anti-PKC iota Picoband antibody, IHC(P): Mouse Brain Tissue.

Anti-PKC iota Picoband antibody, IHC(P): Rat Brain Tissue.

Quick Database Links

Gene Symbol

Protein kinase C iota type

UniProt

P41743