PRKCDBP Antibody

SKU: orb1249468

Description

PRKCDBP Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in Human Adipose, in Mouse Ovary and Rat Uterus and in NIH3T3 lysates (calculated MW of 27.7kDa according to Human NP_659477.2, of 27.8kDa according to Mouse NP_082720.1 and of 27.9kDa according to Rat NP_604444.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ). Recommended concentration: 0.1-1µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-RRTGLQKVQSLR
• Target: PRKCDBP
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRKCDBP, protein kinase C, delta binding protein, HSRBC, MGC20400, SRBC, sdr-related gene product that binds to c-kinase, cavin-3

orb1249468 (0.1 ug/ml) staining of Human Adipose cell llysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249468 (0.3 ug/ml) staining of Mouse Ovary (A) and Rat Uterus (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249468 (3.75 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

PRKCDBP

UniProt

Q969G5

RefSeq (Protein):NP_659477.2