PRKCDBP/CAVIN3 Antibody

• Catalog Number: orb1539304
• Category: Antibodies
• Description: PRKCDBP/CAVIN3 Antibody
• Target: PRKCDBP / CAVIN3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Equine, Hamster, Human, Monkey, Porcine
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: Peptide with sequence C-RRTGLQKVQSLR, from the internal region of the protein sequence according to NP_659477.2.
• Tested applications: ELISA, IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000), WB (0.1 - 1 µg/ml)
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 38kDa band observed in Human Adipose and NIH3T3 lysates (calculated MW of 27.7kDa according to Human NP_659477.2 and 27.8kDa according to Mouse NP_082720.1 ). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ). Recommended concentration: 0.1-1 ug/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PRKCDBP, CAVIN3, HSRBC, SRBC, Cavin-3
• Note: For research use only

PRKCDBP antibody (0.3 ug/ml) staining of Mouse Ovary (A) and Rat Uterus (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.