PRKAR2A Antibody

SKU: orb1274495

Description

PRKAR2A Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: KO/KD Validated, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:1000 - 1:2000

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PRKAR2A (NP_004148.1).
• Target: PRKAR2A
• Molecular Weight: Observed: 46kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PKR2, PRKAR2, cAMP-dependent protein kinase type II-alpha regulatory subunit, cAMP-dependent protein kinase regulatory subunit RII alpha, protein kinase A, RII-alpha subunit, protein kinase, cAMP-dependent, regulatory subunit type II alpha

Western blot analysis of extracts of various cell lines, using PRKAR2A antibody (orb1274495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Western blot analysis of extracts from normal (control) and PRKAR2A knockout (KO) HeLa cells, using PRKAR2A antibody (orb1274495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Quick Database Links

Gene Symbol

PRKAR2A

UniProt

P13861