Rabbit PRKAR2A/PKA-RIIalpha Antibody

SKU: orb1527989

Description

PRKAR2A/PKA-RIIalpha Antibody

Research Area

Cancer Biology, Cardiovascular Research, Immunology & Inflammation, Metabolism Research, Neuroscience

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution Range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Between 50 and 100
• Target: PRKAR2A/PKA-RIIalpha
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Liquid
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase type II-alpha regulatory subunit; PKR2; PRKAR2; protein kinase A, RII-alpha subunit; protein kinase, cAMP-dependent, regulatory subunit type II alpha

Detection of human PRKAR2A/PKA-RIIalpha by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PRKAR2A/PKA-RIIalpha antibody orb1527989 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 30 seconds.

Detection of human PRKAR2A/PKA-RIIalpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PRKAR2A/PKA-RIIalpha antibody orb1527989 used for IP at 3 µg/mg lysate.

Detection of human PRKAR2A/PKA-RIIalpha by immunohistochemistry.Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-PRKAR2A/PKA-RIIalpha (Cat. No. orb1527989) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

PRKAR2A/PKA-RIIalpha

UniProt

P13861

RefSeq (Protein):NP_004148.1