PRKAA2 Antibody

SKU: orb1248881

Description

PRKAA2 Antibody

Images & Validation

• Tested Applications: EIA, ELISA, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 65kDa band observed in Human Heart and Liver lysates and in Mouse and Rat Heart lysates (calculated MW of 62.3kDa according to NP_006243.2). Recommended concentration: 0.3-1µg/mL. Primary incubation was 1 hour.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PRKAA2 captured by a rabbit antibody. Recommended reporter concentration: 1.5-3µg/mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CPLDALNTTKP
• Target: PRKAA2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PRKAA2, protein kinase, AMP-activated, alpha 2 catalytic subunit, AMPK, AMPK2, PRKAA, 5'-AMP-activated protein kinase, catalytic alpha-2 chain, AMP-activated protein kinase alpha 2 catalytic subunit, AMPK-alpha-2 chain

orb1248881 (1.5 ug/ml) as the reporter antibody.

orb1248881 (1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248881 (0.3 ug/ml) staining of Mouse (A) and Rat (B) Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PRKAA2

UniProt

P54646

RefSeq (Protein):NP_006243.2