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PRDX1 Antibody

SKU: orb1247400

Description

PRDX1 Antibody

Research Area

Cancer Biology, Cell Biology

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 23 to 24kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 22.1kDa according to NP_859048.1). Recommended concentration: 0.01-0.03µg/mL.Immunohistochemistry:Paraffin embedded Human Thyroid Gland and Placenta. Recommended concentration: 2.5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-SDPKRTIAQDYG
TargetPRDX1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

PRDX1, peroxiredoxin 1, MSP23, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2, natural killer-enhancing factor A, proliferation-associated gene A, thioredoxin-dependent peroxide reductase 2

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PRDX1 Antibody

orb1247400 (0.01 ug/ml) staining of HepG2 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PRDX1 Antibody

orb1247400 (2.5 ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

PRDX1 Antibody

orb1247400 (2.5 ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Datasheet
Product Information
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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PRDX1 Antibody (orb1247400)

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Available Sizes

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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