Peroxiredoxin 1 Rabbit Polyclonal Antibody

SKU: orb6796

Description

Peroxiredoxin 1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PRDX1 (1-100/199aa)
• Target: PRDX1
• Molecular Weight: 22 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Peroxiredoxin-1; Peroxiredoxin 1; Prdx 1; Prdx1; PRDX1_HUMAN; Hbp23; Heme binding 23 kDa protein; Macrophage 23 Kd stress protein; Macrophage 23kDa stress protein; Macrophase stress protein 22kDa; Macrophase stress protein 23 kd; MSP23; Natural killer cell enhancing factor A; Natural killer cell-enhancing factor A; Natural killer enhancing factor A; NKEF A; NKEF-A; NkefA; OSF3; Osteoblast specific factor 3; PAG; Paga; PAGB; Proliferation associated gene A; Proliferation associated protein PAG; Proliferation-associated gene protein; PrxI; Tdpx 2; Tdpx2; TDX2; Thioredoxin dependent peroxide reductase 2; Thioredoxin peroxidase 2; Thioredoxin-dependent peroxide reductase 2; TPxA; Trx dependent peroxide reductase 2.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Peroxiredoxin 1 antibody (orb6796), dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Sample: Lane 1: Human Raji cell lysates, Lane 2: Human Hela cell lysates, Lane 3: Human A431 cell lysates, Lane 4: Human Hela cell lysates, Primary: Anti-Peroxiredoxin 1 (orb6796) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 22 kD, Observed band size: 23 kD.

Tissue/Cell: Rat brain, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PRDX1 Polyclonal Antibody, Unconjugated (orb6796) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

PRDX1

UniProt

Q06830