PRDM16 Antibody

• Catalog Number: orb1239909
• Category: Antibodies
• Description: PRDM16 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-PRDM16 antibody was raised against a peptide corresponding to 17 amino acid synthetic peptide near the carboxy terminus of human PRDM16. The immunogen is located within amino acids 1120-1170 of PRDM16.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 140kD, 138kD, 120 kD Observed: 130 kD
• Target: PRDM16
• UniProt ID: Q9HAZ2
• NCBI: CAH71530
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PRDM16 Antibody: MEL1, LVNC8, PFM13, CMD1LL, KIAA1
• Note: For research use only
• Application notes: WB: 1-4 μg/mL; IHC-P: 1-2 μg/mL; IHC-P/IF: 10-20 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in the mouse sample. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

WB Validation in MCF7 Cells. Loading: 15 μg of lysate. Antibodies: PRDM16, orb1239909, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Lung. Loading: 15 μg of lysate. Antibodies: PRDM16, orb1239909, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Spleen. Loading: 15 μg of lysate. Antibodies: PRDM16, orb1239909, 2 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of PRDM16 in Human Kidney. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRDM16 antibody (orb1239909) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of PRDM16 in Human Brain. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PRDM16 antibody (orb1239909) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of PRDM16 in Mouse Kidney. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRDM16 antibody (orb1239909) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of PRDM16 in Rat Spleen. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-PRDM16 antibody (orb1239909) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of PRDM16 in Mouse Brain. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling PRDM16 with orb1239909 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).