PRDM16 Antibody (Center)

SKU: orb1926922

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:2000
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB49517
• Immunogen: This PRDM16 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 771-804 amino acids from the Central region of human PRDM16.
• Target: PRDM16 (HGNC:14000)
• Molecular Weight: 140251 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1, PRDM16, KIAA1675, MEL1, PFM13

Anti-PRDM16 Antibody (Center) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 140 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PRDM16 Antibody (Center) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 140 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from LNCaP cell line, mouse lung tissue lysate (from left to right), using PRDM16 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.

Quick Database Links

Gene Symbol

PRDM16 (HGNC:14000)

UniProt

Q9HAZ2