PRAM1 Antibody

• Catalog Number: orb1247078
• Category: Antibodies
• Description: PRAM1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Porcine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: DFCDPLENQPLPLGR
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3ug/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: PRAM1
• UniProt ID: Q96QH2
• NCBI: NP_115528.4
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: PRAM-1, PRAM-1 protein, PML-RARA target gene encod
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3ug/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.
• Expiration Date: 12 months from date of receipt.

orb1247078 staining (2 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.