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PRAM1 Antibody

SKU: orb18326

Description

Goat polyclonal to PRAM1

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:8000, WB: 1-3 μg/ml
ReactivityHuman
Predicted ReactivityPorcine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3 μg/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.

Key Properties

ClonalityPolyclonal
TargetPRAM1
Protein SequenceDFCDPLENQPLPLGR
Molecular Weight74
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti PRAM-1 antibody, anti PRAM-1 protein antibody, anti PML-RARA target gene encoding an Adaptor Molecule-1 antibody, anti PML-RARA regulated adaptor molecule 1 antibody, anti MGC39864 antibody, anti PML-RAR antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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