PPP3CA Mouse Polyclonal Antibody

SKU: orb10217

Description

PPP3CA Mouse Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=2ug/Test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PPP3CA (15-80/521aa)
• Target: PPP3CA
• Molecular Weight: 57 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ACCIID; CALN; CALNA; CALNA1; CCN1; CNA1; DEE91; IECEE; IECEE1; PPP2B; 2900074D19Rik; CN; CnA; PP2BA_HUMAN; PPP3CA; CAM-PRP catalytic subunit; Calcineurin A alpha; Calmodulin-dependent calcineurin A subunit alpha isoform (CNA alpha); Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; 3.1.3.16; PP2BA_MOUSE; PP2BA_RAT;

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PPP3CA) polyclonal Antibody, Unconjugated (orb10217) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-PPP3CA antibody (orb10217): 2 µg/10^6 cells, Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC: 1 µg/test. Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

PPP3CA

UniProt

Q08209