PPP3CA Mouse Polyclonal Antibody

• Catalog Number: orb10217
• Category: Antibodies
• Description: PPP3CA Mouse Polyclonal Antibody
• Species/Host: Mouse
• Clonality: Polyclonal
• Tested applications: FC, ICC
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human PPP3CA (15-80/521aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: ICC/IF=1:25, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 57 kDa
• Target: PPP3CA
• UniProt ID: Q08209
• RRID: AB_10746652
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Calcineurin A alpha; CalcineurinA; Calcineurin-A;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PPP3CA) polyclonal Antibody, Unconjugated (orb10217) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

The MCF-7 (H) cells were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.). Primary Antibody (green): Mouse Anti-PPP3CA antibody (orb10217): 2 µg/10^6 cells, Secondary Antibody (white blue): Goat anti-Mouse IgG-FITC: 1 µg/test. Isotype Control (orange): Mouse IgG. Blank control (black): PBS. Acquisition of 20000 events was performed.