PPP2R5A Antibody

• Catalog Number: orb1247082
• Category: Antibodies
• Description: PPP2R5A Antibody
• Target: PPP2R5A
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-AYNMHSILSNTSAE
• UniProt ID: Q15172
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 55kDa band observed in human heart and skeletal muscle lysate (calculated MW of 56.2kDa according to NP_006234.1). Recommended for use at 0.5-2ug/ml.Immunohistochemistry:In paraffin embedded Human Heart shows textured cytoplasm staining in cardiomyocytes. Recommended concentration, 5-10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: PPP2R5A, protein phosphatase 2, regulatory subunit
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_006234.1

orb1247082 staining (0.5 ug/ml) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247082 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.