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PPP2CA/PPP2CB Antibody

SKU: orb1247916

Description

PPP2CA/PPP2CB Antibody

Images & Validation

Tested ApplicationsELISA, WB
ReactivityHuman, Mouse, Porcine, Rat
Predicted ReactivityBovine, Canine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 36kDa band observed in Human Brain (Cerebellum), Mouse, Rat and Pig Brain lysates (calculated MW of 35.6kDa according to Human NP_002706.1, Mouse NP_062284.1, Rat NP_058735.1 and Pig NP_999531.1). Recommended concentration: 0.05-0.2ug/ml.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-EPHVTRRTPDYFL
TargetPPP2CA / PPP2CB
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
DisclaimerFor research use only

Alternative Names

PPP2CA, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, PP2Ac, PP2CA, RP-C, PP2A-alpha, protein phosphatase 2, catalytic subunit, alpha isoform, replication protein C, serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PPP2CB, protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform, PP2CB, PP2A-beta, protein phosphatase 2, catalytic subunit, beta isoform, protein phosphatase type 2A catalytic subunit, serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform

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PPP2CA/PPP2CB Antibody

orb1247916 (0.05 ug/ml) staining of Human Cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PPP2CA/PPP2CB Antibody

orb1247916 (0.1 ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PPP2CA/PPP2CB Antibody (orb1247916)

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0.1 mg
$ 920.00
DispatchUsually dispatched within 5-10 working days
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