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PPP2CA/PPP2CB Antibody

SKU: orb18321

Description

Goat polyclonal antibody to PPP2CA / PPP2CB

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml
ReactivityHuman, Mouse, Porcine, Rat
Predicted ReactivityBovine, Canine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human Brain (Cerebellum), Mouse Brain and Rat Brain lysates (calculated MW of 35.6kDa according to NP_002706.1and NP_004147.1). Recommended concentration: 0.05-0.2 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetPPP2CA / PPP2CB
Protein SequenceEPHVTRRTPDYFL
Molecular Weight35.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti PPP2CA antibody, anti protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform antibody, anti PP2Ac antibody, anti PP2CA antibody, anti RP-C antibody, anti PP2A-alpha antibody, anti protein phosphatase 2, catalytic subunit, alpha isoform antibody, anti replication protein C antibody, anti serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform antibody, anti serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody, anti PPP2CB antibody, anti protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform antibody, anti PP2CB antibody, anti PP2A-beta antibody, anti protein phosphatase 2, catalytic subunit, beta isoform antibody, anti protein phosphatase type 2A catalytic subunit antibody, anti serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform antibody

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PPP2CA/PPP2CB Antibody

Western blot analysis of Mouse (Lane1), Rat (Lane2) and Pig (Lane3) Brain lysates using PPP2CA / PPP2CB antibody

PPP2CA/PPP2CB Antibody

Western blot analysis of Human Cerebellum lysate using PPP2CA / PPP2CB antibody

PPP2CA/PPP2CB Antibody

Immunohistochemical staining of Human Kidney of PPP2CA / PPP2CB antibody.

PPP2CA/PPP2CB Antibody

Immunohistochemical staining of Human Thyroid of PPP2CA / PPP2CB antibody.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PPP2CA/PPP2CB Antibody (orb18321)

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Available Sizes

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100 μg
$ 530.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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