PPHLN1 Antibody

SKU: orb1539399

Description

PPHLN1 Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, IF (5 µg/ml), IHC, IHC-P (10 µg/ml), WB (1 µg/ml)
• Reactivity: Human, Mouse
• Application Notes:
  Further information: Periphilin antibody can be used for detection of Periphilin by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A 16 AA synthetic peptide near the center of human Periphilin. The immunogen is located within aa 160-210 of Periphilin.
• Target: PPHLN1
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PPHLN1, Gastric cancer antigen Ga50, HSPC232, HSPC206, Periphilin 1, Periphilin-1

Anti-PPHLN1 antibody IHC staining of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Immunofluorescence of Periphilin in human colon tissue with Periphilin antibody at 5 µg/mL.

Western blot analysis of Periphilin in mouse colon tissue lysate with Periphilin antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Quick Database Links

Gene Symbol

PPHLN1