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PPARA Antibody
Description
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB: 1/500 - 1/2000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Monkey |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 5D10C3 |
| Immunogen | Purified recombinant fragment of human PPARA (AA: 1-120) expressed in E. Coli. |
| Target | FDA approved drug target related |
| Molecular Weight | 52.2kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−PPAR alpha Rabbit Polyclonal Antibody [orb15031]
FC, IF, IHC-Fr, IHC-P, WB
Bovine, Equine, Gallus, Porcine, Rabbit
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μlPPAR-α Polyclonal Antibody [orb1412440]
IF, IHC-P, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μl

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Western blot analysis of human recombinant protein using PPARA antibody

Western blot analysis of HEK293 using PPARA antibody

Western blot analysis of HepG2 (Lane1), HEK293 (Lane2), COS7 (Lane7), HepG2 (Lane4), and Jurkat (Lane5) cell lysate using PPARA antibody

ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

Flow cytometric analysis of Jurkat cells using PPARA antibody
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Protocol Information
PPARA Antibody (orb333984)
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