Ppara Antibody

SKU: orb345413

Description

PPAR alpha antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:75,000 - 1:125,000, IHC: 1:100-1:300, IF: 1-5µg/mL, WB: 1:500 - 1:2,000
• Reactivity: Human, Mouse
• Application Notes:
  Anti-PPAR alpha Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Immunofluorescence. Expect a single band approximately 52 kDa in size corresponding to PPAR alpha by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: PPAR alpha Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.
• Target: Ppara
• Purity: Anti-PPAR alpha Antibody is directed against mouse PPAR alpha protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, rat, bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha protein from human, chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Ppar alpha antibody, Pparα, Peroxisome proliferator-activated receptor alpha, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Ppar-a, Nr1c1, Ppar

Quick Database Links

Gene Symbol

Ppara

UniProt

P23204

RefSeq:31543500