PPAR delta Antibody

SKU: orb345412

Description

PPAR Delta antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:30,000 - 1:70,000, IHC: 1:500, WB: 1:500 - 1:5,000
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 43 kDa in size corresponding to PPAR delta by western blot in the appropriate tissue or cell lysate.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-PPAR delta antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta.
• Purity: This affinity purified antibody is directed against mouse PPAR delta protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with PPAR delta protein from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-PPAR delta antibody, PPARD, PPARB, NR1C2, PPAR-delta, NUCI, Nuclear hormone receptor 1 antibody, Nuclear receptor subfamily 1 group C member 2 antibody, Nuclear hormone receptor 1, NUC-1, Peroxisome proliferative activated receptor delta antibody, Peroxisome proliferator-activated receptor beta, PPAR-beta

Affinity Purified Rabbit Anti-PPAR delta is shown to detect a predominant band at ~ 40 kDa corresponding to PPAR delta present in mouse heart whole cell lysates (p/n orb348716). Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 µg of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO (p/n orb348624) in PBS overnight at 4°C followed reaction with a 1:10000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) for 45 min at room temperature (800 nm channel, green).

ELISA results of purified Rabbit anti-PPAR Delta (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1 µg of conjugate. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) (p/n orb347654) and TMB ELISA Peroxidase Substrate (p/n orb348651).

Immunohistochemistry of Rabbit Anti-PPAR delta (N terminal specific). Antigen Retrieval: HIER pH6.2. [Right] -Neg Ctrl: Normal rabbit IgG on heart striated muscle, pH6.2 (40X). [Left Top - low mag, Left Bottom - 20X] -Staining: Diffuse cytoplasmatic positivity for PPAR delta in human myocardiocitys at 1:500.

Upregulation of lipogenic genes and down-regulation of lipolytic genes associated with NASH induction and aggravation. Wild type (Wt), myeloid IKKβ deficient (IkbkbΔmye), or hepatocyte IKKβ deficient (IkbkbΔhep) mice in both genders, were fed regular chow (Control) or high cholesterol and saturated fat diet (HCFD) from 2.5 months of age for 20 weeks. (A) Immunoblotting of whole liver lysate and (B) densitometric analysis of pAMPK/AMPK, nSREBP-1c, PPARδ, pJNK-1/JNK-1, and pJNK-2/JNK-2. (C) Plasma adiponectin levels measured by ELISA. (D) Real-time PCR results of PPAR α, δ, and γ genes. *P < 0.05, **P < 0.01, ***P < 0.001 compared to Control diet within gender and within genotype. †P < 0.05, ††P < 0.01 compared to other genotype within gender and within diet.

Quick Database Links

UniProt

P35396

RefSeq:548577