PPAR Delta antibody

• Catalog Number: orb345412
• Category: Antibodies
• Description: PPAR Delta antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Anti-PPAR delta antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse PPAR delta.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:30,000 - 1:70,000, IHC: 1:500, WB: 1:500 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against mouse PPAR delta protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat sources based on 100% homology for the immunogen sequence. Cross-reactivity with PPAR delta protein from human, chimpanzee and rabbit may occur as this sequence shows 85% homology with the protein from these sources. Cross-reactivity with PPAR delta homologues from other sources has not been determined. No reactivity is expected against other subtypes of PPAR.
• Conjugation: Unconjugated
• UniProt ID: P35396
• NCBI: 548577
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-PPAR delta antibody, PPARD, PPARB, NR1
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a single band approximately 43 kDa in size corresponding to PPAR delta by western blot in the appropriate tissue or cell lysate.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse heart whole cell lysates using PPAR Delta antibody