PP5 Antibody: Biotin

• Catalog Number: orb377031
• Category: Antibodies
• Description: Mouse monoclonal antibody against PP5 conjugated to Biotin.
• Target: PP5
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Immunogen: Full length human PP5 protein
• UniProt ID: P53041
• MW: 58kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Application notes: 1 µg/ml of SMC-244 was sufficient for detection of PP5 in 15 µg of human A431 lysates by ECL analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: 12F7
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: PPP5 antibody, PPT antibody, Protein phosphatase T
• Note: For research use only
• Entrez: 5536
• NCBI: NP_006238.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.

Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Lane 1: MW Ladder. Lane 2: Human A431 (15 μg). Lane 3: Human HEK293 (15 μg). Lane 4: Human Jurkat (15 μg). Load: 15 μg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.