FKBP51 Antibody: Biotin

• Catalog Number: orb147194
• Category: Antibodies
• Description: Mouse monoclonal to FKBP51 (Biotin). Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins. FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90. Although FKBP52 and FKBP51 share 75% sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics. FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids..
• Target: FKBP51
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Reactivity: Canine, Hamster, Human, Mouse, Rabbit, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Immunogen: Synthetic peptide corresponding to the residues of human FKBP51
• UniProt ID: Q13451
• MW: 51kDa
• Tested applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:2000), ICC/IF (1:1000)
• Application notes: A 1:2000 dilution was sufficient for detection of FKBP51 in ~50 µg total protein using WB analysis.
• Clone Number: Hi51B
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: AIG6 antibody, FK506 binding protein 5 antibody, F
• Note: For research use only
• Entrez: 2289
• NCBI: NP_001139247.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B. Tissue: MK cells. Species: Mouse. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. Cells stained red.

Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of FKBP51 protein using Mouse Anti-FKBP51 Monoclonal Antibody, Clone Hi51B. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-FKBP51 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.