POU5F1 Antibody

SKU: orb1249113

Description

POU5F1 Antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 38-40kDa band observed in lysates of cell line NIH3T3 and approx. 45kDa in lysates of cell line HepG2 (calculated MW of 38.6kDa according to Human NP_002692.2 and 38.2kDa according to Mouse NP_038661.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-2µg/mL.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-DRPNAVKLEKVEP
• Target: POU5F1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Pou5f1, POU domain, class 5, transcription factor 1, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4

Quick Database Links

Gene Symbol

POU5F1

UniProt

P20263

RefSeq (Protein):NP_038661.2