POU5F1 Antibody

SKU: orb1239199

Description

POU5F1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Gallus, Porcine
• Application Notes:
  POU5F1 antibody can be used for detection of POU5F1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. Antibody validated: Western Blot in mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: POU5F1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human POU5F1.The immunogen is located within amino acids 260 - 310 of POU5F1.
• Target: POU5F1
• Purification: POU5F1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: POU5F1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

POU5F1 Antibody: OCT3, OCT4, OTF3, OTF4, OTF-3, Oct-3, Oct-4, OCT3, POU domain, class 5, transcription factor 1, Octamer-binding protein 3, 41915

Western blot analysis of POU5F1 in mouse liver tissue lysate with POU5F1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of POU5F1 in rat liver tissue with POU5F1 antibody at 5 µg/mL.

Quick Database Links

Gene Symbol

POU5F1

UniProt

Q01860

RefSeq (Protein):NP_002692