Plk rabbit pAb

• Catalog Number: orb769495
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Plk
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human PLK1. AA range:176-225
• MW: 70
• Tested applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-PLK1 antibody, anti-PLK antibody, anti-Serine
• Note: For research use only

Immunofluorescence analysis of A549 cells, using PLK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Western blot analysis of lysates from LOVO cells, treated with PMA 125ng/ml 30', using PLK1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).