PLEK/Pleckstrin Antibody

SKU: orb1538013

Description

PLEK/Pleckstrin Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: IF (10 µg/ml), IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:16000), WB (0.01 - 0.3 µg/ml)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 3.75 ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-EWIKAIQMASRTGK, from the C-Terminus of protein sequence according to NP_002655.2.
• Target: PLEK / Pleckstrin
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PLEK, Platelet 47 kDa protein, Pleckstrin, p47

Pleckstrin Antibody Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Pleckstrin Antibody staining (0.01 µg/ml) of Human Parathyroid lysate (A) and negative control HepG2 (B) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescencence.

Pleckstrin Antibody Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton.

Quick Database Links

Gene Symbol

PLEK / Pleckstrin