PKC ε rabbit pAb

• Catalog Number: orb769656
• Category: Antibodies
• Description: Rabbit polyclonal antibody to PKC Epsilon
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PKC epsilon. AA range:688-737
• Concentration: 1 mg/ml
• Dilution range: WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
• Conjugation: Unconjugated
• MW: 83
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-PRKCE antibody, anti-PKCE antibody, anti-Prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot analysis of 453 cells using PKC ε Polyclonal Antibody diluted at 1:500.

Western blot analysis of lysates from HeLa, Jurkat, and 3T3 cells, treated with PMA 125ng/ml 30', using PKC epsilon Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).