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| Catalog Number | orb769655 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to PKC Epsilon (phospho-Ser729) |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PKC epsilon around the phosphorylation site of Ser729. AA range:688-737 |
| Concentration | 1 mg/ml |
| Dilution range | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Conjugation | Unconjugated |
| MW | 83 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-PRKCE antibody, anti-PKCE antibody, anti-Prot Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC epsilon (Phospho-Ser729) Antibody.
Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using PKC epsilon (Phospho-Ser729) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
