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Catalog Number | orb1258413 |
---|---|
Category | Antibodies |
Description | PITX2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PITX2 (NP_000316.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:100 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 39kDa |
Target | PITX2 |
UniProt ID | Q99697 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | PITX2, RS, RGS, ARP1, Brx1, IDG2, IGDS, IHG2, PTX2 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:100 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using PITX2 antibody (orb1258413) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
IF, WB | |
Gallus, Mouse, Rat, Xenopus, Zebrafish | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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