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PITX2 Antibody (C-term)

Catalog Number: orb1935940

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1935940
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB10568
Tested applicationsIF, WB
Predicted ReactivityMouse, Other, Rat, Zebrafish
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeIF: 1:10~50, WB: 1:2000, WB: 1:2000
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW35370 Da
TargetThis PITX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-151 amino acids of human PITX2.
UniProt IDQ99697
NCBINP_700476.1, NP_700475.1, NP_001191326.1, NP_001191327.1, NP_001191328.1, NP_000316.2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesPituitary homeobox 2, ALL1-responsive protein ARP1
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NoteFor research use only
Expiration Date12 months from date of receipt.
PITX2 Antibody (C-term)

All lanes: Anti-Pilx2 (Human C-term) at 1:2000 dilution. Lane 1: CCRF-CEM whole cell lysate. Lane 2: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PITX2 Antibody (C-term)

Anti-Pilx2 (Human C-term) at 1:2000 dilution + K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 35 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

PITX2 Antibody (C-term)

Fluorescent image of Hela cell stained with PITX2 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PITX2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). PITX2 immunoreactivity is localized to Nucleus significantly.