PIR Antibody

SKU: orb1247097

Description

PIR Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 33kDa band observed in Human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). Recommended concentration: 0.3 - 1.0 µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Spleen. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: GSSKKVTLSVLSREC
• Target: PIR
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PIR, pirin, pirin (iron-binding nuclear protein)

Quick Database Links

Gene Symbol

PIR

UniProt

O00625

RefSeq (Protein):NP_003653.1