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Catalog Number | orb18865 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to PIR |
Target | Pirin |
Clonality | Polyclonal |
Species/Host | Goat |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Protein Sequence | GSSKKVTLSVLSREC |
RRID | AB_10755538 |
MW | 32.1 |
Tested applications | ELISA, IHC, WB |
Dilution range | ELISA: 1:128000, WB: 0.3 - 1.0 μg/ml, IHC-P: 5 μg/ml |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 33kDa band observed in human Heart lysate (calculated MW of 32.1kDa according to NP_003653.1). In transfected HEK293 transiently expressing PIR a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3 - 1.0 μg/ml. |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti pirin antibody, anti PIR antibody, anti pirin Read more... |
Note | For research use only |
Entrez | 8544 |
Western blot analysis of Human Heart lysate using PIR antibody
Western blot analysis of HEK293 lysate using PIR antibody
Immunohistochemical staining of Human Liver using PIR antibody
Immunohistochemical staining of Human Spleen using PIR antibody