PIGY Antibody

SKU: orb1239228

Description

PIGY Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y.The immunogen is located within amino acids 50 - 100 of PIG-Y.
• Target: PIGY
• Molecular Weight: Predicted: 8 kDa Observed: 16 kDa
• Purification: PIG-Y Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PIG-Y Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PIG-Y Antibody: PIG-Y, Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein, PIG-Y

Western blot analysis of PIG-Y in human liver tissue lysate with PIG-Y antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of PIG-Y in human spleen tissue with PIG-Y antibody at 2.5 µg/mL.

Immunofluorescence of PIGY in human spleen tissue with PIGY1 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

PIGY

UniProt

Q3MUY2

RefSeq (Protein):NP_116295