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Phosphotyrosine Antibody / P-Tyr

Catalog Number: orb640115

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb640115
CategoryAntibodies
DescriptionProtein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Species/HostMouse
ClonalityMonoclonal
Clone NumberPY265
Tested applicationsFACS, IF, WB
ReactivityOther
IsotypeMouse IgG1, kappa
ImmunogenPhosphotyrosine was used as the immunogen for the Phosphotyrosine antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis Phosphotyrosine antibody is available for research use only.
StorageStore the Phosphotyrosine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
Phosphotyrosine Antibody / P-Tyr

SDS-PAGE analysis of purified, BSA-free Phosphotyrosine antibody (clone PY265) as confirmation of integrity and purity.

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    FACS,  IF,  IHC-P,  IP,  WB

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    ELISA

    All

    Rabbit

    Recombinant

    Unconjugated

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