Phosphotyrosine Antibody / P-Tyr

• Catalog Number: orb640132
• Category: Antibodies
• Description: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: PY2870R
• Tested applications: ELISA
• Reactivity: All
• Isotype: Rabbit IgG, kappa
• Immunogen: Phosphotyrosine was used as the immunogen for the recombinant Phosphotyrosine antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA (order BSA-free format for coating)
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant Phosphotyrosine antibody is available for research use only.
• Storage: Store the recombinant Phosphotyrosine antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE analysis of purified, BSA-free Phosphotyrosine antibody (clone PY2870R) as confirmation of integrity and purity.